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& Rosenfeld, R

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ICH Q2(R2) explains general principles for checking whether an analytical method is suitable and reliable

Excessive or poorly targeted antioxidant intervention could theoretically alter normal signalling as well as damaging oxidation

Glutathione is an antioxidant which is found in all the cells in our body, providing protection from free radicals and peroxides

Following incubation, caspase 3 activity was measured using the fluorogenic substrate AC-DEVD-AMC as described before ( 2 , 1.2 mM EGTA, 1.5% Nonidet P40, 0.3% CHAPS, 30% sucrose, 150 M caspase 3 substrate DEVD-AMC, 30 mM DTT, and 3.0 mM PMSF was directly added to HepG2 cells