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When combining the 90% sensitivity cutoff (0.01 pg ml 1 ) with the 90% specificity cutoff (0.02 pg ml 1 ) in a two-cutoff approach, the NPV of the lower cutoff was, as above, 0.833 (95% CI 0.7130.910) and the PPV of the upper cutoff was 0.884 (95% CI 0.7890.940), reaching an overall accuracy for those below the lower cutoff and above the upper cutoff of 86.2% (95% CI 78.891.7%), with 53 of the 176 individuals falling in the intermediate zone, which corresponded to 30.1% of the evaluated participants

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