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Isolated cells were activated with 5 g/ml plate-bound CD3, 2 g/ml soluble CD28 (eBioscience, San Diego, CA), and 200 units/ml IL-2 (TECIN) and treated with 0-1000 nM tirzepatide for 3 days in RPMI supplemented with 10% FBS, 300 mg/L L-glutamine, 100 units/mL penicillin, 100 g/mL streptomycin, 1mM sodium pyruvate, 100 M NEAA, 1 mM HEPES, 55 M -mercaptoethanol, and 0.2% plasmocin mycoplasma prophylactic

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Sustained Treatment: Participants in studies achieved a significant weight reduction of 20.9% after 36 weeks of taking Tirzepatide at the maximum tolerated dose