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A final protective layer of low melting-point agarose was applied, coverslipped, and allowed to solidify for 10 min at 4 C before coverslip removal

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In hypogonadal men with diabetes or in male rodents with androgen deficiency, decreased mitochondrial density, abnormal mitochondrial morphology, and lower ATP production were observed, while exogenous testosterone supplementation effectively alleviated mitochondrial dysfunction and improved glucose and lipid metabolism ( 2 The sex-dimorphic regulatory impact of androgens and AR on glucose and lipid metabolism 2.1 Inhibited androgens/AR negatively affects glucose and lipid metabolism in males The most well-known androgens include testosterone, androstenedione, dehydroepiandrosterone (DHEA), dehydroepiandrosterone sulfate (DHEA-S), and dihydrotestosterone (DHT)

REVIEW OF THE LITERATURE Potential therapeutic mechanisms of N-acetylcysteine NAC possesses multiple beneficial pharmacological properties, e.g., anti-inflammatory, antioxidant, minimises cytokine action, modulation release of dopamine, reversing of mitochondrial malfunction, diminishes apoptosis and ferroptosis, improved neurogenesis and boosted glutamate release

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